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This paper presents a complete and accurate procedure for the determination of the dissociation constants of vitamin B12 (cobalamin) and its complex with methylcobalamin (MeCbl), in aqueous solution, by HPLC on octylsulfonamide (OSA) bonded silica gel columns at different pH values. The method is based on the use of cobalamin and MeCbl (or holo-MeCbl) as carriers of a previously synthesized ion-pair, by displacement of the different ionizable groups present on the mentioned cobalamins (anionic and/or zwitterionic). The products of the assays are collected on a barrier which is eluted from the column in order to quantify the dissociation constants using the retention times of the standards and the quality of the chromatographic peaks. The reproducibility of the determination of the dissociation constants (obtained by measuring seven solutions with the same composition, obtaining a RSD lower than 1.5%) is also assayed. Results show high values of Kc, as expected, suggesting a high degree of ionization of all cobalamins forms. Our methodology has been used to determine the Kc for cobalamin and MeCbl as well as for a stock solution of vitamin B12 and of cyanocobalamin (CN-Cbl).HTC is already turning around its fortunes in the smartphone sector, this summer’s release of the One A9 handsets in Europe is a strong first step in that direction. But just how does one go about turning back a years-long slide in the record books? After all, HTC in the United States (and around the globe) has sold fewer than a million devices per quarter since the massive phone leak in late 2013. The numbers do seem to be changing, however, with last week’s announcement that the manufacturer sold over 2 million HTC One A9s in the first three months of this year. The HTC One M9/M9+
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